Lue Y A, Ellner P D, Ellner D A
Sex Transm Dis. 1980 Oct-Dec;7(4):165-7.
Five thousand eight hundred forty-nine cervical and rectal swabs were cultured for Neisseria gonorrhoeae. Swabs remained in Amies' transport medium for approximately 2 hr before primary plating and enrichment for 6 hr in a selective broth. Neisseria gonorrhoeae was detected in 87 specimens. In 72 (83%) of the 87, N. gonorrhoeae was detected by primary plating, and in 15 (17%), detection was possible only after enrichment. Although enrichment is advantageous in recovering N. gonorrhoeae from swabs that have been in transport medium for several hours, the advantage of the enrichment technique over immediate direct plating remains to be determined. Positive enrichment broth cultures were tested at 6 hr for the presence of N. gonorrhoeae; 52% were positive by counterimmunoelectrophoresis, 71% were positive by coagglutination, and 97% were positive by enzyme-linked immunosorbent assay.
对5849份宫颈和直肠拭子进行淋病奈瑟菌培养。拭子在Amies运送培养基中保存约2小时后进行初次接种，并在选择性肉汤中增菌6小时。在87份标本中检测到淋病奈瑟菌。在这87份标本中的72份（83%），通过初次接种检测到淋病奈瑟菌，15份（17%）仅在增菌后才检测到。尽管增菌有利于从在运送培养基中放置数小时的拭子中回收淋病奈瑟菌，但增菌技术相对于立即直接接种的优势仍有待确定。对增菌肉汤培养阳性的标本在6小时时检测是否存在淋病奈瑟菌；对流免疫电泳法阳性率为52%，协同凝集法阳性率为71%，酶联免疫吸附测定法阳性率为97%。
