一种用于检测生殖器和生殖器外标本中淋病奈瑟菌的实时聚合酶链反应检测方法。
A real-time PCR assay for the detection of Neisseria gonorrhoeae in genital and extragenital specimens.
作者信息
Whiley David M, Buda Philip P, Freeman Kevin, Pattle Neville I, Bates John, Sloots Theo P
机构信息
Clinical Virology Research Unit, Sir Albert Sakzewski Virus Research Centre, Royal Children's Hospital and Health Service District, Queensland 4029, Australia.
出版信息
Diagn Microbiol Infect Dis. 2005 May;52(1):1-5. doi: 10.1016/j.diagmicrobio.2004.12.011.
A Neisseria gonorrhoeae LightCycler (NGpapLC) assay targeting the porA pseudogene was compared with bacterial culture for detection of N. gonorrhoeae in 636 clinical specimens (216 cervical, 185 urethral, 196 throat, and 39 rectal swab specimens). The specificity of the NGpapLC assay was further investigated by testing a bacterial reference panel comprising several Neisseria species. Overall, 19 (3.0%) specimens were positive and 613 (96.4%) specimens were negative by both methods. Four (0.6%) specimens were positive by the NGpapLC assay only. For the cervical and urethral swabs, the NGpapLC provided 100% sensitivity and 100% specificity compared with bacterial culture. Following discrepant analysis, the clinical sensitivity and specificity of the NGpapLC for throat and rectal swabs was also 100%. For the bacterial panel, only N. gonorrhoeae isolates provided positive results. The results show the NGpapLC assay is suitable for use on a range of clinical specimens and could improve detection of pharyngeal N. gonorrhoeae.
将针对porA假基因的淋病奈瑟菌LightCycler（NGpapLC）检测法与细菌培养法进行比较，以检测636份临床标本（216份宫颈标本、185份尿道标本、196份咽喉标本和39份直肠拭子标本）中的淋病奈瑟菌。通过检测包含几种奈瑟菌属的细菌参考面板，进一步研究了NGpapLC检测法的特异性。总体而言，两种方法检测出19份（3.0%）标本呈阳性，613份（96.4%）标本呈阴性。4份（0.6%）标本仅通过NGpapLC检测法呈阳性。对于宫颈和尿道拭子，与细菌培养法相比，NGpapLC检测法的灵敏度和特异性均为100%。经过差异分析后，NGpapLC检测法对咽喉和直肠拭子的临床灵敏度和特异性也均为100%。对于细菌参考面板，只有淋病奈瑟菌分离株给出了阳性结果。结果表明，NGpapLC检测法适用于一系列临床标本，并且可以提高咽部淋病奈瑟菌的检测率。
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