戊二醛与人红细胞的反应。

Reaction of glutaraldehyde with human erytrocytes.

作者信息

de Carvalho J G, Osório e Castro V R, Perrone J C

出版信息

An Acad Bras Cienc. 1980 Jun;52(2):419-26.

Abstract

The reaction of glutaraldehyde with human erythrocytes has been studied at pH values of 6.0, 7.2 and 8.0 at several glutaraldehyde concentrations, for different periods at 37 degrees C. Increasing pH and reagent concentration favours formation of cells resistant to osmotic and chemical (by n-butanol, saponin and sodium dodecyl sulfate (SDS)) hemolysis. Ultrasonic waves also have no effect upon these cells. Different behavior concerning the state of hemoglobin, K+ efflux, and swelling have also been observed, according to the pH of treatment. The results suggest that crosslinking involving hemoglobin molecules and components of the membrane (proteins, aminophospholipids) may be obtained in the reaction of human erythrocytes with glutaraldehyde.

摘要

在37摄氏度下，于不同时间段，研究了在pH值为6.0、7.2和8.0时，几种戊二醛浓度下戊二醛与人红细胞的反应。pH值和试剂浓度的增加有利于形成对渗透和化学（通过正丁醇、皂角苷和十二烷基硫酸钠（SDS））溶血具有抗性的细胞。超声波对这些细胞也没有影响。根据处理的pH值，还观察到了关于血红蛋白状态、钾离子外流和肿胀的不同行为。结果表明，在人红细胞与戊二醛的反应中，可能会发生涉及血红蛋白分子和膜成分（蛋白质、氨基磷脂）的交联。