Changes in the serine-specific transfer ribonucleic acid pattern of guinea pig epidermis after corticosteroid treatment.

The pattern of serine-specific tRNAs from guinea pig epidermis was determined and compared to that from liver by employing reversed phase chromatography on total tRNA preparations aminoacylated with 3H- or 14C-serine. Five tRNAserS (I-V) were found, two of which, tRNAserI and tRNAserV, appear to be "typical" for epidermis and in so far probably reflect in some way metabolic peculiarities of epidermal cell differentiation. This hypothesis was further corroborated by showing that the locally applied corticosteroid triamcinolon selectively increases tRNAserI and tRNAserV 2-3-fold. The implications of these findings were discussed in detail.