Charles M A, Martial J, Zolock D, Morrissey R, Baxter J D
Calcif Tissue Int. 1981;33(1):15-8. doi: 10.1007/BF02409407.
1,25-Dihydroxycholecalciferol modulates the synthesis of the intestinal calcium-binding protein. To determine if this effect is due to an increase in calcium-binding protein mRNA activity, we measured total cytoplasmic protein-specific mRNA activity from chick intestine in an in vitro wheat germ translation system. 1,25-Dihydroxycholecalciferol enhances calcium-binding protein specific mRNA activity. The sterol does not induce a general increase in mRNA synthesis since the concentration of only a few proteins increased. Thus 1,25-dihydroxycholecalciferol regulates only a subset of genes and specifically affects a transcriptional process involving calcium-binding protein synthesis.
1,25 - 二羟基胆钙化醇调节肠钙结合蛋白的合成。为了确定这种作用是否归因于钙结合蛋白信使核糖核酸（mRNA）活性的增加，我们在体外麦胚翻译系统中测量了来自鸡肠道的总细胞质蛋白特异性mRNA活性。1,25 - 二羟基胆钙化醇增强了钙结合蛋白特异性mRNA活性。该固醇并不会导致mRNA合成普遍增加，因为只有少数蛋白质的浓度增加了。因此，1,25 - 二羟基胆钙化醇仅调节一部分基因，并特异性地影响涉及钙结合蛋白合成的转录过程。
