Japha J L, Eder T J, Goldsmith E D
Am J Anat. 1977 May;149(1):23-8. doi: 10.1002/aja.1001490103.
The pineal gland of the Mongolian gerbil consists of a superficial gland, stalk and deep pineal. The deep pineal differentiates postnatally. Histochemical studies of the superficial pineal gland indicate that it may be involved in the secretion of protein. Presumptive secretory material visualized by aldehyde fuchsin (AF) and chrome hematoxylin was observed along the course of blood vessels and among the pinealocytes. The distribution and texture of the AF-positive material was distinctive. It did not correspond to the pattern and texture of material stained with PAS, Sudan Black or acid orcein. Staining with AF was markedly reduced after incubation with trypsin, indicating that the AF-positive material is at least partially protein. The amount of stainable material increased with age. The AF-positive material was observed in what appeared to be interstitial or glial cells and processes, and in the processes of perivascular cells. Cells and fibrous processes with high non-specific esterase activity ("high-esterase cells") were observed among the pinealocytes and along the course of blood vessels. The distribution of the "high-esterase cells" and the morphology and texture of their esterase-containing processes were remarkably similar to the morphology and distribution of the material that stained with AF. It may be that the "high-esterase cells" contain AF-positive material. The "high-esterase cells" hydrolyzed both alpha-naphthyl acetate and alpha-naphthyl butyrate. The pinealocytes hydrolyzed only alpha-naphthyl acetate. The "high-esterase cells" appear to form a distinct class of cells within the superficial pineal gland. They are tentatively identified as a type of glial cell.
蒙古沙鼠的松果体由浅表腺体、柄和深部松果体组成。深部松果体在出生后分化。对浅表松果体的组织化学研究表明，它可能参与蛋白质的分泌。通过醛复红（AF）和铬苏木精观察到的推定分泌物质沿血管行程和松果体细胞之间可见。AF阳性物质的分布和质地独特。它与用PAS、苏丹黑或酸性orcein染色的物质的模式和质地不对应。用胰蛋白酶孵育后，AF染色明显减少，表明AF阳性物质至少部分是蛋白质。可染色物质的量随年龄增加。在似乎是间质或神经胶质细胞及其突起以及血管周围细胞的突起中观察到AF阳性物质。在松果体细胞之间和血管行程中观察到具有高非特异性酯酶活性的细胞和纤维突起（“高酯酶细胞”）。“高酯酶细胞”的分布及其含酯酶突起的形态和质地与用AF染色的物质的形态和分布非常相似。可能“高酯酶细胞”含有AF阳性物质。“高酯酶细胞”能水解醋酸α-萘酯和丁酸α-萘酯。松果体细胞仅能水解醋酸α-萘酯。“高酯酶细胞”似乎在浅表松果体内形成一类独特的细胞。它们被初步鉴定为一种神经胶质细胞类型。
