Kitagawa Y, Okuhara E
J Biochem. 1980 Dec;88(6):1607-13. doi: 10.1093/oxfordjournals.jbchem.a133136.
The rabbit antiserum against poly(I).poly(C) purified by hydroxyapatite column chromatography contained three distinct antibodies. They were fractionated into three antibody populations by a series of precipitations (with poly(A).poly(U), poly(I), and poly(I).poly(C)) and their specificities were examined by quantitative complement fixation, double diffusion tests and radioimmunoassay. The first population was common to the double helical structure of double-stranded RNAs. The second was specific for poly(I) and the third was specific for poly(I).poly(C). These studies demonstrated that specific antibodies exclusively reactive with poly(I).poly(C) existed in the rabbit antiserum against poly(I).poly(C).
通过羟基磷灰石柱色谱法纯化的抗聚肌苷酸-聚胞苷酸兔抗血清含有三种不同的抗体。通过一系列沉淀（用聚腺苷酸-聚尿苷酸、聚肌苷酸和聚肌苷酸-聚胞苷酸）将它们分离成三个抗体群体，并通过定量补体结合、双向扩散试验和放射免疫测定法检测它们的特异性。第一个群体对双链RNA的双螺旋结构具有普遍性。第二个群体对聚肌苷酸具有特异性，第三个群体对聚肌苷酸-聚胞苷酸具有特异性。这些研究表明，在抗聚肌苷酸-聚胞苷酸兔抗血清中存在仅与聚肌苷酸-聚胞苷酸反应的特异性抗体。
