Payne C C, Kalmakoff J
J Immunol Methods. 1975 Dec;9(2):147-56. doi: 10.1016/0022-1759(75)90105-2.
An antiserum was prepared in rabbits to the synthetic double-stranded ribonucleic acid (ds RNA) poly rI:rC. Using a liquid-phase radioimmunoassay, the antiserum cross-reacted with a natural ds RNA isolated from the cytoplasmic polyhedrosis virus of the silkworm, binding 95% of the RNA at a 1 : 20 serum dilution. Preliminary tests of the specificity of the antiserum showed that it did not bind single-stranded RNA (ss RNA) or deoxyribonucleic acid (DNA), but also revealed that the serum contained an enzyme activity which degraded ss RNA into acid-insoluble fragments. It was therefore possible that the failure to bind ss RNA resulted from the degradation of the antigen rather than from an absence of cross-reacting antibodies. However, when the serum ribonuclease activity was inhibited by macaloid, the antiserum still did not bind the ss RNA antigen. This demonstrated that the antibodies to ds RNA did not cross-react with ss RNA. The existence of serum enzymes capable of degrading nucleic acid antigens emphasizes the need for caution in assessing the specificity of such antisera.
用合成的双链核糖核酸（ds RNA）多聚 rI：rC 在兔子体内制备了抗血清。使用液相放射免疫测定法，该抗血清与从家蚕细胞质多角体病毒中分离出的天然 ds RNA 发生交叉反应，在血清稀释度为 1:20 时能结合 95%的 RNA。对抗血清特异性的初步测试表明，它不与单链 RNA（ss RNA）或脱氧核糖核酸（DNA）结合，但也显示该血清含有一种酶活性，可将 ss RNA 降解为酸不溶性片段。因此，未能结合 ss RNA 可能是由于抗原的降解，而不是由于缺乏交叉反应抗体。然而，当血清核糖核酸酶活性被绿坡缕石抑制时，抗血清仍然不与 ss RNA 抗原结合。这表明针对 ds RNA 的抗体不与 ss RNA 发生交叉反应。血清中存在能够降解核酸抗原的酶，这强调了在评估此类抗血清特异性时需要谨慎。
