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巨大芽孢杆菌QM b1551几种新型噬菌体的宿主范围及部分特性研究

Host-range and partial characterization of several new bacteriophages for Bacillus megaterium QM b1551.

作者信息

Vary P S, Halsey W F

出版信息

J Gen Virol. 1980 Nov;51(Pt 1):137-46. doi: 10.1099/0022-1317-51-1-137.

Abstract

Several phages infecting Bacillus megaterium QM B1551 have been isolated from the soil and partially characterized. These phages, designated MP9 to MP50, were tested for host-range on several strains of B. megaterium and 13 other Bacillus species. All the phages only infected B. megaterium and on the basis of host-range patterns, 23 groups could be distinguished. The phage patterns also distinguished subgroups of B. megaterium strains within the species and should be useful in phage typing. The phages have varying sensitivities to heat, salts and organic solvents and are all double-stranded DNA phages. Thirty-two have been examined by electron microscopy and are Bradley types A, B and C. This is the first large collection of B. megaterium phages that has been characterized.

摘要

已从土壤中分离出几种感染巨大芽孢杆菌QM B1551的噬菌体,并对其进行了部分特性鉴定。这些噬菌体被命名为MP9至MP50,在几种巨大芽孢杆菌菌株和其他13种芽孢杆菌属物种上测试了宿主范围。所有噬菌体仅感染巨大芽孢杆菌,根据宿主范围模式可区分出23个组。噬菌体模式还区分了该物种内巨大芽孢杆菌菌株的亚组,应有助于噬菌体分型。这些噬菌体对热、盐和有机溶剂具有不同的敏感性,均为双链DNA噬菌体。通过电子显微镜检查了32种,它们属于布拉德利A、B和C型。这是首次对大量巨大芽孢杆菌噬菌体进行特性鉴定。

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