Popp A, Hertwig S, Lurz R, Appel B
Department of Biological Safety, Robert Koch-Institut, Berlin, Germany.
Syst Appl Microbiol. 2000 Dec;23(4):469-78. doi: 10.1016/S0723-2020(00)80020-X.
170 Yersinia strains belonging to various species were investigated for the presence of temperate bacteriophages. By induction with mitomycin C seven phages were isolated from Y. enterocolitica strains and one phage from a Y. frederiksenii strain. The phages were characterized on the basis of their morphology, host range, genome size, DNA homology, and protein composition. They belong to different phage families and reveal narrow to moderate wide host ranges. Some of the isolated phages were able to infect pathogenic as well as nonpathogenic strains of Y. enterocolitica. The genomes of all isolated phages were found to be composed of double stranded DNA ranging from about 40 to 60 kb. In addition to the analysed phages, a number of putative phages were induced in strains of Y. frederiksenii, Y. kristensenii, Y. intermedia, and Y. mollaretii. The putative phages were identified by isolation of phage DNA from cell free lysates but could not be propagated on indicator strains. Southern hybridization experiments revealed relationships between phages belonging to different families. Moreover, DNA homologies were observed between phages isolated from nonpathogenic Yersinia strains and a phage which was isolated from a pathogenic Y. enterocolitica serogroup O:3 strain.
对170株属于不同种的耶尔森氏菌菌株进行了温和噬菌体存在情况的研究。通过用丝裂霉素C诱导，从小肠结肠炎耶尔森氏菌菌株中分离出7种噬菌体，从费氏耶尔森氏菌菌株中分离出1种噬菌体。根据噬菌体的形态、宿主范围、基因组大小、DNA同源性和蛋白质组成对其进行了表征。它们属于不同的噬菌体家族，宿主范围从窄到中等宽。一些分离出的噬菌体能够感染小肠结肠炎耶尔森氏菌的致病菌株和非致病菌株。所有分离出的噬菌体的基因组均由约40至60 kb的双链DNA组成。除了分析的噬菌体外，在费氏耶尔森氏菌、克氏耶尔森氏菌、中间耶尔森氏菌和莫氏耶尔森氏菌菌株中还诱导出了一些推定的噬菌体。通过从无细胞裂解物中分离噬菌体DNA鉴定出了推定的噬菌体，但它们不能在指示菌株上增殖。Southern杂交实验揭示了不同家族噬菌体之间的关系。此外，在从非致病性耶尔森氏菌菌株分离出的噬菌体与从致病性小肠结肠炎耶尔森氏菌O:3血清群菌株分离出的一种噬菌体之间观察到了DNA同源性。
