噬菌体SPP1 DNA的膜结合
Membrane binding of bacteriophage SPP1 DNA.
作者信息
Burger K J
出版信息
Mol Gen Genet. 1980;179(2):373-6. doi: 10.1007/BF00425467.
PMID:6780755
Abstract
A fast sedimenting complex was isolated from B. subtilis cells infected with bacteriophage SPP1 by renografin centrifugation. This complex was identified as membrane bound parental and replicating SPP1 DNA. Synthesis of SPP1 DNA takes place in close association with the membrane. This newly synthesized DNA is then released into the cytoplasm. During release, concatemeric SPP1 DNA is sized into monomeric DNA molecules.
摘要
通过泛影葡胺离心法从感染噬菌体SPP1的枯草芽孢杆菌细胞中分离出一种快速沉降复合物。该复合物被鉴定为与膜结合的亲本和正在复制的SPP1 DNA。SPP1 DNA的合成与膜紧密相关。然后，这种新合成的DNA被释放到细胞质中。在释放过程中，串联的SPP1 DNA被切割成单体DNA分子。
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