枯草芽孢杆菌染色体的多个区域与细胞膜的关联。
Association of many regions of the Bacillus subtilis chromosome with the cell membrane.
作者信息
Ivarie R D, Pène J J
出版信息
J Bacteriol. 1973 May;114(2):571-6. doi: 10.1128/jb.114.2.571-576.1973.
Abstract
Unsheared lysates of Bacillus subtilis 168T(-) containing uniformly labeled deoxyribonucleic acid (DNA) were exposed to varying doses of gamma rays to introduce double-strand scissions in the chromosome. From an estimate of the number-average molecular weight and the amount of DNA bound to membrane after irradiation, about 70 to 90 regions of the bacterial chromosome were detected in membrane fractions. Since this number was independent of the molecular weight of the DNA (i.e., the extent of fragmentation of the chromosome), it is thought to represent an upper limit in the number of membrane-binding sites per chromosome.
摘要
将含有均匀标记脱氧核糖核酸（DNA）的枯草芽孢杆菌168T（-）未剪切裂解物暴露于不同剂量的γ射线下，以在染色体中引入双链断裂。通过对辐照后数均分子量和与膜结合的DNA量的估计，在膜组分中检测到约70至90个细菌染色体区域。由于该数字与DNA的分子量无关（即染色体的片段化程度），因此认为它代表了每条染色体上膜结合位点数量的上限。
相似文献
1
Association of many regions of the Bacillus subtilis chromosome with the cell membrane.枯草芽孢杆菌染色体的多个区域与细胞膜的关联。
J Bacteriol. 1973 May;114(2):571-6. doi: 10.1128/jb.114.2.571-576.1973.
2
Topography of chromosome membrane junction in Bacillus subtilis.枯草芽孢杆菌中染色体膜连接的拓扑结构。
Nat New Biol. 1973 Aug 15;244(137):204-6. doi: 10.1038/newbio244204a0.
3
Association of the Bacillus subtilis chromosome with the cell membrane: resolution of free and bound deoxyribonucleic acid on renografin gradients.枯草芽孢杆菌染色体与细胞膜的关联：在泛影葡胺梯度上分离游离和结合的脱氧核糖核酸
J Bacteriol. 1970 Nov;104(2):839-50. doi: 10.1128/jb.104.2.839-850.1970.
4
Neutral sucrose gradient sedimentation of very large DNA from Bacillus subtilis. II. Double-strand breaks formed by gamma ray irradiation of the cells.枯草芽孢杆菌超大DNA的中性蔗糖梯度沉降。II. 细胞经γ射线照射形成的双链断裂
J Mol Biol. 1973 Apr 15;75(3):479-94. doi: 10.1016/0022-2836(73)90455-5.
5
Chromosome-membrane association in Bacillus subtilis. I. DNA release from membrane fraction.枯草芽孢杆菌中的染色体-膜关联。I. 从膜组分中释放DNA。
Biochem Biophys Res Commun. 1971 Sep 17;44(6):1559-65. doi: 10.1016/s0006-291x(71)80264-4.
6
Neutral sucrose sedimentation of very large DNA from Bacillus subtilis. I. Effect of random double-strand breaks and centrifuge speed on sedimentation.枯草芽孢杆菌超大DNA的中性蔗糖沉降。I. 随机双链断裂和离心速度对沉降的影响。
J Mol Biol. 1973 Apr 15;75(3):455-78. doi: 10.1016/0022-2836(73)90454-3.
7
Membrane attachment of the replication origins of a multifork (dichotomous) chromosome in Bacillus subtilis.枯草芽孢杆菌中多叉（二分体）染色体复制起点的膜附着
J Mol Biol. 1972 Aug 21;69(2):237-48. doi: 10.1016/0022-2836(72)90228-8.
8
Membrane attachment of the chromosome replication origin in Bacillus subtilis.枯草芽孢杆菌中染色体复制起点的膜附着
Cold Spring Harb Symp Quant Biol. 1968;33:695-705. doi: 10.1101/sqb.1968.033.01.078.
9
Characterization of the Bacillus subtilis W23 genome by sedimentation.通过沉降法对枯草芽孢杆菌W23基因组进行表征。
J Mol Biol. 1972 Dec 30;72(3):801-6. doi: 10.1016/0022-2836(72)90192-1.
10
Gamma-ray induced strand breakage of Bacillus subtilis DNA irradiated in vivo.体内照射的枯草芽孢杆菌DNA的γ射线诱导链断裂
Can J Microbiol. 1971 May;17(5):575-83. doi: 10.1139/m71-095.
引用本文的文献
1
Membrane binding of bacteriophage SPP1 DNA.噬菌体SPP1 DNA的膜结合
Mol Gen Genet. 1980;179(2):373-6. doi: 10.1007/BF00425467.
2
Heterospecific transformation in Bacillus subtilis: protein composition of a membrane-DNA complex containing unstable heterologous donor-recipient complex.枯草芽孢杆菌中的异种特异性转化：一种包含不稳定异源供体 - 受体复合物的膜 - DNA 复合物的蛋白质组成
Mol Gen Genet. 1984;197(3):478-85. doi: 10.1007/BF00329946.
3
Molecular fate of heterologous bacterial DNA in competent Bacillus subtilis: further characterization of unstable association between donor and recipient DNA and the involvement of the cellular membrane.感受态枯草芽孢杆菌中异源细菌DNA的分子命运：供体与受体DNA之间不稳定关联的进一步表征及细胞膜的作用
Mol Gen Genet. 1984;195(1-2):200-8. doi: 10.1007/BF00332747.
4
Identification of specific restriction fragments associated with a membrane subparticle from Bacillus subtilis.与枯草芽孢杆菌膜亚颗粒相关的特定限制性片段的鉴定。
J Bacteriol. 1983 Jun;154(3):1389-96. doi: 10.1128/jb.154.3.1389-1396.1983.
5
Isopycnic centrifugation of chromatin in renografin solutions.在泛影葡胺溶液中对染色质进行等密度离心。
J Cell Biol. 1974 Jun;61(3):780-8. doi: 10.1083/jcb.61.3.780.
6
Effect of rifampin on the structure and membrane attachment of the nucleoid of Escherichia coli.利福平对大肠杆菌类核结构及膜附着的影响。
J Bacteriol. 1973 Dec;116(3):1364-74. doi: 10.1128/jb.116.3.1364-1374.1973.
7
Membrane attachment of the chromosome in Bacillus subtilis mutants temperature-sensitive in DNA replication.枯草芽孢杆菌中对DNA复制温度敏感的突变体的染色体膜附着
Nucleic Acids Res. 1974 Dec;1(12):1775-82. doi: 10.1093/nar/1.12.1775.
8
Interactions between exogenous deoxyribonucleic acid and membrane vesicles isolated from Bacillus subtilis 168.外源脱氧核糖核酸与枯草芽孢杆菌168分离的膜囊泡之间的相互作用。
J Bacteriol. 1974 Sep;119(3):784-94. doi: 10.1128/jb.119.3.784-794.1974.
9
Rejoining of radiation-induced single-strand breaks in deoxyribonucleic acid of Escherichia coli: effect of phenethyl alcohol.大肠杆菌脱氧核糖核酸中辐射诱导单链断裂的重新连接：苯乙醇的作用
J Bacteriol. 1975 Jan;121(1):392-5. doi: 10.1128/jb.121.1.392-395.1975.
10
Nuclear and cell division in Bacillus subtilis: cell development from spore germination.枯草芽孢杆菌中的核与细胞分裂：从孢子萌发开始的细胞发育
J Bacteriol. 1976 Apr;126(1):384-99. doi: 10.1128/jb.126.1.384-399.1976.
本文引用的文献
1
Sedimentation rate as a measure of molecular weight of DNA.沉降速率作为DNA分子量的一种衡量指标。
Biophys J. 1963 Jul;3(4):309-21. doi: 10.1016/s0006-3495(63)86823-x.
2
Intracellular location and number of replicating parental DNA molecules of bacteriophages lambda and phi-X174.噬菌体λ和φ-X174复制亲代DNA分子的细胞内定位及数量
J Mol Biol. 1969 Apr 14;41(1):39-65. doi: 10.1016/0022-2836(69)90124-7.
3
Production and repair of radiochemical damage in Escherichia coli deoxyribonucleic acid; its modification by culture conditions and relation to survival.大肠杆菌脱氧核糖核酸中放射化学损伤的产生与修复；其受培养条件的影响及与存活的关系
J Bacteriol. 1971 Jan;105(1):127-35. doi: 10.1128/jb.105.1.127-135.1971.
4
DNA-strand scission and loss of viability after x irradiation of normal and sensitized bacterial cells.正常细菌细胞和致敏细菌细胞经X射线照射后的DNA链断裂及生存力丧失
Proc Natl Acad Sci U S A. 1966 Jun;55(6):1442-6. doi: 10.1073/pnas.55.6.1442.
5
DNA-membrane association and the repair of double breaks in x-irradiated Micrococcus radiodurans.X射线辐照的耐辐射微球菌中DNA与膜的结合及双链断裂的修复
Biochim Biophys Acta. 1971 Sep 30;247(1):38-53. doi: 10.1016/0005-2787(71)90805-7.
6
Competence for genetic transformation and the release of DNA from Bacillus subtilis.枯草芽孢杆菌的遗传转化能力及DNA释放
Biochim Biophys Acta. 1971 Feb 25;232(1):61-71. doi: 10.1016/0005-2787(71)90491-6.
7
Association of the Bacillus subtilis chromosome with the cell membrane: resolution of free and bound deoxyribonucleic acid on renografin gradients.枯草芽孢杆菌染色体与细胞膜的关联：在泛影葡胺梯度上分离游离和结合的脱氧核糖核酸
J Bacteriol. 1970 Nov;104(2):839-50. doi: 10.1128/jb.104.2.839-850.1970.
8
Spontaneous release of DNA in sequential genetic order by Bacillus subtilis.枯草芽孢杆菌按顺序遗传顺序自发释放DNA。
J Mol Biol. 1969 Oct 14;45(1):137-52. doi: 10.1016/0022-2836(69)90216-2.
9
Membrane attachment of the chromosome replication origin in Bacillus subtilis.枯草芽孢杆菌中染色体复制起点的膜附着
Cold Spring Harb Symp Quant Biol. 1968;33:695-705. doi: 10.1101/sqb.1968.033.01.078.
10
Evidence for the involvement of membranous bodies in the processes leading to genetic transformation in Bacillus subtilis.关于膜状体参与枯草芽孢杆菌遗传转化过程的证据。
J Bacteriol. 1966 Oct;92(4):1111-21. doi: 10.1128/jb.92.4.1111-1121.1966.
Zotero 插件