Kato T, Seiji M
Tohoku J Exp Med. 1980 Apr;130(4):311-20. doi: 10.1620/tjem.130.311.
The smooth endoplasmic reticulum (smooth-surface membrane, SSM) is quite similar in properties to the cytoplasmic which is known to be antigenic. SSM of the melanoma melanocytes can be isolated from the mouse melanoma as a reasonably pure preparation and it contains tyrosinase. Rabbits were immunized by the SSM isolated from Harding-Passey mouse melanomas. Formation of the antibody was detected by the double immuno-diffusion method. Anti-SSM-serum thus formed was found to react with cytoplasmic membrane in the immunofluorescent test, and also showed the specific cytotoxicity to the mouse melanoma cells. On the other hand, the tyrosinase activity of SSM was not inhibited by this antiserum.
光滑内质网（光滑表面膜，SSM）在性质上与已知具有抗原性的细胞质非常相似。黑色素瘤黑素细胞的SSM可以从小鼠黑色素瘤中分离出来，制成相当纯的制剂，并且它含有酪氨酸酶。用从哈丁 - 帕西小鼠黑色素瘤中分离出的SSM免疫兔子。通过双向免疫扩散法检测抗体的形成。发现由此形成的抗SSM血清在免疫荧光试验中与细胞质膜发生反应，并且对小鼠黑色素瘤细胞也表现出特异性细胞毒性。另一方面，这种抗血清不会抑制SSM的酪氨酸酶活性。
