Gulmen S
J Oral Pathol. 1976 Nov;5(6):325-33. doi: 10.1111/j.1600-0714.1976.tb01784.x.
The antigenic potential of spontaneous malignant melanoma of the Syrian hamster was investigated. Antisera to the tumor were prepared in two groups of rabbits; one group was immunized with tumor homogenate material and the other with tissue cultured tumor cells. The antiserum was absorbed with normal hamster tissue extracts and serum to remove cross-reacting antibodies. The presence of tumor specific antibodies was demonstrated using immunodiffusion and fluorescent antibody staining techniques. Tumor specific antiserum prepared in this manner gave a single precipitin line against tumor tissue in gel-diffusion tests, and showed specific staining of tumor cells in the fluorescent antibody test.
对叙利亚仓鼠自发性恶性黑色素瘤的抗原潜力进行了研究。在两组兔子中制备了针对该肿瘤的抗血清；一组用肿瘤匀浆材料免疫，另一组用组织培养的肿瘤细胞免疫。用正常仓鼠组织提取物和血清吸收抗血清以去除交叉反应抗体。使用免疫扩散和荧光抗体染色技术证明了肿瘤特异性抗体的存在。以这种方式制备的肿瘤特异性抗血清在凝胶扩散试验中针对肿瘤组织产生单一沉淀线，并在荧光抗体试验中显示肿瘤细胞的特异性染色。
