Soergel M E, Schaffer F L, Sawyer J C, Prato C M
Arch Virol. 1978;57(3):271-82. doi: 10.1007/BF01315091.
A radioimmune assay method designated St-RIP using a staphylococcal IgG adsorbent, which potentially has broad applications to viral (and nonviral) antigen-antibody systems, was applied to detection of calicivirus antibodies. Purified 125I-labeled virions of San Miguel sea lion virus serotypes 4 (SMSV-4) and 5 (SMSV 5) were incubated with sera; the immune complexes were reacted with an immunoadsorbent, formaldehyde-fixed staphylococci (Staphylococcus aureus protein A producer, strain Cowan I), and collected by centrifugation. Broad cross-reactivity was observed among serotypes of SMSV and vesicular exanthema of swine virus (VESV), but there was no reaction with antisera to six noncaliciviruses. Antibody production in a rabbit inoculated with SMSV-5 polypeptide was monitored by St-RIP assay; reactivity with intact SMSV-4 virion antigen was slightly less than, but closely paralleled, reactivity with SMSV-5 virion antigen. Applicability of the St-RIP test to serologic survey was demonstrated with pinniped, swine, and human (laboratory personnel) sera; numerous positive St-RIP reactions suggested the occurrence of widespread contacts with caliciviruses.
一种使用葡萄球菌IgG吸附剂的放射免疫分析方法，即葡萄球菌放射免疫沉淀法（St-RIP），该方法可能广泛应用于病毒（和非病毒）抗原-抗体系统，被用于检测杯状病毒抗体。将纯化的125I标记的圣米格尔海狮病毒4型（SMSV-4）和5型（SMSV-5）病毒粒子与血清一起孵育；免疫复合物与免疫吸附剂（甲醛固定的葡萄球菌，金黄色葡萄球菌蛋白A产生菌，考恩I株）反应，并通过离心收集。在SMSV血清型和猪水疱性疹病毒（VESV）之间观察到广泛的交叉反应，但与六种非杯状病毒的抗血清没有反应。通过St-RIP分析监测接种SMSV-5多肽的兔子体内的抗体产生；与完整的SMSV-4病毒粒子抗原的反应性略低于与SMSV-5病毒粒子抗原的反应性，但密切平行。通过对鳍足类动物、猪和人类（实验室人员）血清的检测，证明了St-RIP试验在血清学调查中的适用性；大量阳性的St-RIP反应表明存在与杯状病毒的广泛接触。
