Romppanen T, Viljanen M K, Sorvari T E
Immunology. 1981 Mar;42(3):391-9.
Colchicine was applied on the anal lips of chickens on 4 consecutive days after hatching. At the ages of 4 and 10 weeks the agglutinin responses to Brucella abortus and to sheep red blood cells (SRBC) after intraperitoneal and per anum immunization were decreased in these animals. IgG and IgM antibodies to Brucella, measured by enzyme-linked immunosorbent assay (ELISA), were also diminished in 12-week-old chickens. The total concentration of plasma IgG, measured by the ELISA-inhibition method, was diminished whereas the IgM level was not influenced by the colchicine treatment. These results broadly resemble the findings after surgical bursectomy or cyclophosphamide treatment. It can be concluded that colchicine applied on the anal lips of chickens causes a long-lasting deficiency of humoral immunity and thus seems to be a useful new tool in the exploration of the humoral-immune functions of the chickens.
在雏鸡孵化后的连续4天，将秋水仙碱涂抹于其肛门唇。在4周龄和10周龄时，这些动物经腹腔和经肛门免疫后，对流产布鲁氏菌和绵羊红细胞（SRBC）的凝集素反应降低。通过酶联免疫吸附测定（ELISA）检测，12周龄鸡体内针对布鲁氏菌的IgG和IgM抗体也减少。采用ELISA抑制法测定的血浆IgG总浓度降低，而IgM水平不受秋水仙碱处理的影响。这些结果与手术切除法氏囊或环磷酰胺处理后的结果大致相似。可以得出结论，涂抹于雏鸡肛门唇的秋水仙碱会导致体液免疫长期缺陷，因此似乎是探索雏鸡体液免疫功能的一种有用的新工具。
