Dubey J P
J Am Vet Med Assoc. 1981 Apr 1;178(7):700-3.
Ten 75- to 105-day-pregnant does each were inoculated orally within 1 million (2 does), 10,000 (4 does), or 1,000 (4 does) sporocysts of Sarcocystis from coyote feces. Two does not inoculated with sporocysts served as controls. The 2 does inoculated with 1 million sporocysts died from acute sarcocystosis 21 and 22 days after inoculation (DAI), and each had 2 dead fetuses. The 4 does inoculated with 10,000 sporocysts were ill 19 to 33 DAI but survived; 1 aborted at 33 DAI, 1 had a live kid that died within 2 hours of birth 31 DAI, 1 aborted 2 dead fetuses 23 DAI, and 1 had a normal kid 56 DAI. The 4 does inoculated with 1,000 sporocysts and the 2 control does remained clinically normal and had normal kids. Does and their offspring were killed within 24 hours of parturition, and their tissues were examined histologically and microbiologically. Meronts of Sarcocystis were found in the maternal placenta of does inoculated with 1 million sporocysts. Sarcocystis was not found in the placenta, fetuses, or tissues of kids from does inoculated with 10,000 or 1,000 sporocysts, or from control does. Other abortifacient agents were not found in the placenta, fetuses, or kids from any does.
选取10只怀孕75至105天的母羊,每只分别经口接种来自郊狼粪便的100万个(2只)、10000个(4只)或1000个(4只)肉孢子虫包囊。2只未接种包囊的母羊作为对照。接种100万个包囊的2只母羊在接种后21天和22天死于急性肉孢子虫病,且每只都有2个死胎。接种10000个包囊的4只母羊在接种后19至33天发病但存活下来;1只在33天流产,1只产下1只活羔羊,该羔羊在31天出生后2小时内死亡,1只在23天流产2个死胎,1只在56天产下1只正常羔羊。接种1000个包囊的4只母羊和2只对照母羊临床症状正常且产下正常羔羊。母羊及其后代在分娩后24小时内被宰杀,对其组织进行组织学和微生物学检查。在接种100万个包囊的母羊的母体胎盘中发现了肉孢子虫裂殖体。在接种10000个或1000个包囊的母羊所产羔羊的胎盘、胎儿或组织中,以及对照母羊所产羔羊的上述部位均未发现肉孢子虫。在任何母羊的胎盘、胎儿或羔羊中均未发现其他流产因子。