Dubey J P
J Am Vet Med Assoc. 1981 Apr 1;178(7):671-4.
Fifteen pregnant goats serologically negative for Toxoplasma gondii were inoculated orally with 10, 100, or 1,000 infective oocysts of the GT-1 strain of T gondii. Four goats received 10 oocysts, 6 received 100 oocysts, and 5 received 1,000 oocysts. Six pregnant goats served as noninoculated controls. All inoculated goats became pyretic (40-41 C) and were dyspneic between 4 and 12 days after inoculation (DAI). Two goats inoculated in early pregnancy (68 and 70 days) with 100 oocysts had enlarged uteri; fetal membranes but no fetuses were found when they were killed 86 and 88 DAI. Three goats aborted or had a stillborn kid 9, 10, and 11 DAI. The remaining 10 goats delivered kids infected with T gondii; 8 goats had at least 1 stillborn kid, and 2 goats had infected but otherwise normal kids at birth. Placenta and tissues of fetuses and kids were inoculated into mice for determination of Toxoplasma infectivity. Toxoplasma gondii was isolated from the fetal placenta of 6 of 7 goats as early as 10 DAI and as late as 62 DAI. The organism was isolated from every fetus or kid that was born 18 DAI but not from fetuses or kids born 9, 10, and 11 DAI. Two kids were born 15 DAI; T gondii was isolated from 1 kid but not from the other, suggesting that T gondii invaded fetal tissues between 11 and 15 DAI. Of the 20 transplacentally infected kids, T gondii was isolated more frequently from the skeletal muscle, heart, lung, and brain than from other tissues.
15只弓形虫血清学检测为阴性的怀孕山羊经口接种10、100或1000个刚地弓形虫GT - 1株感染性卵囊。4只山羊接种10个卵囊，6只接种100个卵囊，5只接种1000个卵囊。6只怀孕山羊作为未接种对照。所有接种山羊在接种后4至12天体温升高（40 - 41摄氏度）且出现呼吸困难。2只在怀孕早期（68和70天）接种100个卵囊的山羊子宫增大；在接种后86和88天处死时发现有胎膜但无胎儿。3只山羊在接种后9、10和11天流产或产出死胎。其余10只山羊产下感染弓形虫的幼崽；8只山羊至少有1只死胎，2只山羊产下感染但其他方面正常的幼崽。将胎儿和幼崽的胎盘及组织接种到小鼠体内以测定弓形虫的感染性。早在接种后10天至晚至62天，从7只山羊中的6只胎儿胎盘分离出了刚地弓形虫。从接种后18天出生的每只胎儿或幼崽中都分离出了该病原体，但未从接种后9、10和11天出生的胎儿或幼崽中分离出。2只幼崽在接种后15天出生；从1只幼崽中分离出了弓形虫，另1只未分离出，这表明弓形虫在接种后11至15天侵入胎儿组织。在20只经胎盘感染的幼崽中，从骨骼肌、心脏、肺和大脑中分离出弓形虫的频率高于其他组织。
