来自芽孢杆菌质粒pAB124的四环素抗性基因会降低枯草芽孢杆菌中抗生素的积累，但不会降低大肠杆菌中抗生素的积累。
Tetracycline resistance genes from Bacillus plasmid pAB124 confer decreased accumulation of the antibiotic in Bacillus subtilis but not in Escherichia coli.
作者信息
Eccles S, Docherty A, Chopra I, Shales S, Ball P
出版信息
J Bacteriol. 1981 Mar;145(3):1417-20. doi: 10.1128/jb.145.3.1417-1420.1981.
Abstract
Expression of tetracycline resistance by genes originating in the Bacillus plasmid pAB124 was examined in both Bacillus subtilis and Escherichia coli host cells. Expression of resistance in B. subtilis by genes from pAB124 was inducible and associated with decreased accumulation of the antibiotic. A fragment of pAB124 carrying the genes coding for tetracycline resistance was cloned into the E. coli plasmid RSF2124. The cloned fragment conferred a low level of resistance in E. coli, but this was not associated with decreased uptake of tetracycline and was not inducible.
摘要
对源自芽孢杆菌质粒pAB124的基因在枯草芽孢杆菌和大肠杆菌宿主细胞中的四环素抗性表达进行了检测。来自pAB124的基因在枯草芽孢杆菌中的抗性表达是可诱导的，并且与抗生素积累的减少相关。将携带编码四环素抗性基因的pAB124片段克隆到大肠杆菌质粒RSF2124中。克隆片段在大肠杆菌中赋予低水平的抗性，但这与四环素摄取的减少无关，并且是不可诱导的。
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