脂质体包裹的质粒DNA向枯草芽孢杆菌原生质体和经钙处理的大肠杆菌细胞的转移。
Transfer of liposome-encapsulated plasmid DNA to Bacillus subtilis protoplasts and calcium-treated Escherichia coli cells.
作者信息
Holubová I, Jandová Z, Tichý P, Hubácek J, Ludvík J
出版信息
Folia Microbiol (Praha). 1985;30(2):97-100. doi: 10.1007/BF02922200.
PMID:3922858
Abstract
Protoplasts of Bacillus subtilis 168 trpC2 str and cells of Escherichia coli SK 1590 after treatment with calcium chloride were transformed to tetracycline resistance with the recombinant plasmid pUN82 entrapped in the reverse phase evaporation liposomes. Frequency of transfer was 4 X 10(-4)% in B. subtilis and 8 X 10(-6)% in E. coli.
摘要
用氯化钙处理后的枯草芽孢杆菌168 trpC2 str原生质体和大肠杆菌SK 1590细胞，被包封在反相蒸发脂质体中的重组质粒pUN82转化为对四环素具有抗性。在枯草芽孢杆菌中的转移频率为4×10⁻⁴%，在大肠杆菌中的转移频率为8×10⁻⁶%。
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