Bell J M, John A M
J Nutr. 1981 Mar;111(3):525-30. doi: 10.1093/jn/111.3.525.
Linear programmed diets designed to maximize the use of proteins and to minimize the use of free (1-) amino acids, and containing five dietary levels of each amino acid under test, were fed to weanling crossbred Carworth Farms No. 1 x Swiss mice in 14-day growth trials. Arginine dietary levels were 0.1, 0.2, 0.3, 0.4 and 0.7%; lysine: 0.2, 0.3, 0.4, 0.5 and 1.0%; tryptophan: 0.03, 0.07, 0.10, 0.13 and 0.17%; and phenylalanine: 0.10, 0.25, 0.40, 0.55 and 0.89%. Growth, feed consumption and regression-adjusted growth rates indicated the following minimum requirements: arginine less than 0.1% and probably zero, lysine 0.4%, tryptophan 0.1% and phenylalanine 0.4%. The AIN '76 reference diet was included in each amino acid test and resulted in superior growth and feed utilization. It was postulated that the greater content of free amino acids in our diets may have affected the efficiency of feed utilization adversely.
为使蛋白质利用最大化并尽量减少游离（1-）氨基酸的使用而设计的线性程序饮食，在14天的生长试验中喂给断奶的杂种卡沃思农场1号×瑞士小鼠，每种受试氨基酸含有五个饮食水平。精氨酸的饮食水平分别为0.1%、0.2%、0.3%、0.4%和0.7%；赖氨酸：0.2%、0.3%、0.4%、0.5%和1.0%；色氨酸：0.03%、0.07%、0.10%、0.13%和0.17%；苯丙氨酸：0.10%、0.25%、0.40%、0.55%和0.89%。生长、饲料消耗和回归调整后的生长率表明了以下最低需求量：精氨酸低于0.1%，可能为零，赖氨酸0.4%，色氨酸0.1%，苯丙氨酸0.4%。每种氨基酸测试中都包含了AIN '76参考饮食，其生长和饲料利用率更高。据推测，我们饮食中较高含量的游离氨基酸可能对饲料利用效率产生了不利影响。
