Peel C J, Taylor J W, Robinson I B, McGowan A A, Hooley R D, Findlay J K
Aust J Biol Sci. 1978 Apr;31(2):187-95. doi: 10.1071/bi9780187.
Lactations were successfully induced in 15 out of 18 non-pregnant cows treated with oestradiol-17beta (0.1 mg/kg body weight) and progesterone (0.25 mg/kg body weight) on days 1-7 (where day 1 is the first day of treatment). A further 23 out of 26 cows were successfully treated with oestradiol-17beta and progesterone standardized at 40 and 100 mg/day respectively. No significant differences in milk yields were obtained by the additional treatments of twice daily milking from day 10, sustained elevation or suppression of plasma prolactin during the induction phase by reserpine and bromo-cryptine respectively, or by continuing oestradiol-17beta injections alone on days 8-11. Levels of plasma prolactin less than 20 ng/ml were adequate for the preparation of the mammary gland for lactation to occur. The induced lactation of 24 monozygotic twin cows ranged from 20 to 87% of their respective siblings which had normal parturient lactations. All 11 cows treated with the standard oestrogen-progesterone treatment plus reserpine had successfully induced lactations. In the absence of oestrogen and progesterone treatment, a short period of resperpine treatment followed by dexamethasone trimethylacetate failed to induce lactation in five cows. Ninety per cent of induced cows (n = 39) and 91% of parturient cows (n = 22) conceived and subsequently calved following natural mating.
在第1 - 7天（第1天为治疗首日）用17β - 雌二醇（0.1毫克/千克体重）和孕酮（0.25毫克/千克体重）治疗的18头未怀孕奶牛中，有15头成功诱导泌乳。另外26头奶牛分别用标准剂量为40毫克/天和100毫克/天的17β - 雌二醇和孕酮成功治疗。从第10天开始每天挤奶两次、在诱导期分别用利血平和溴隐亭持续升高或抑制血浆催乳素、或在第8 - 11天单独继续注射17β - 雌二醇，这些额外处理在产奶量上未获得显著差异。血浆催乳素水平低于20纳克/毫升足以使乳腺为泌乳做好准备。24头单卵双胎奶牛的诱导泌乳量为各自正常分娩泌乳的同胞姐妹的20%至87%。用标准雌激素 - 孕酮治疗加用利血平治疗的所有11头奶牛均成功诱导泌乳。在没有雌激素和孕酮治疗的情况下，先用利血平短期治疗，随后用醋酸三甲基地塞米松治疗，5头奶牛未能诱导泌乳。90%的诱导泌乳奶牛（n = 39）和91%的分娩奶牛（n = 22）在自然交配后受孕并随后产犊。
