Exner T, Rickard K A, Kronenberg H
Aust J Exp Biol Med Sci. 1978 Apr;56(2):169-76. doi: 10.1038/icb.1978.18.
Amphoteric polyelectrolytes with alkaline isoelectric points (for example Ampholine 9-11) intended for use in isoelectric focussing were found to shorten the prolonged partial thrombosplastin clotting time with kaolin (PTTK) of plasma deficient in factor VIII. The response of this PTTK to Ampholine 9-11 was linear when plotted on double log paper with a slope slightly greater than that of factor VIII itself. Ampholine 9-11 at 2% concentration had factor VIII and factor IX activity equal to that of normal plasma as well as somewhat less activity as factor XI. It had no significant correcting effect on the clotting of plasmas deficient in factors V, VII, X, II OR XII. Ampholine 9-11 inhibited low concentrations of contact activators and its effect in correcting factor VIII deficient plasma was found to be related to the degree of contact activation.
用于等电聚焦的具有碱性等电点的两性聚电解质（例如pH 9 - 11两性电解质载体）被发现可缩短缺乏因子VIII的血浆用高岭土测定的延长的部分凝血活酶凝血时间（PTTK）。当在双对数纸上作图时，该PTTK对pH 9 - 11两性电解质载体的反应呈线性，斜率略大于因子VIII本身。2%浓度的pH 9 - 11两性电解质载体具有与正常血浆相等的因子VIII和因子IX活性以及略低于因子XI的活性。它对缺乏因子V、VII、X、II或XII的血浆的凝血没有显著的校正作用。pH 9 - 11两性电解质载体抑制低浓度的接触激活剂，并且发现其校正因子VIII缺乏血浆的作用与接触激活程度有关。
