Cripps A W, Adams D B
Aust J Exp Biol Med Sci. 1978 Apr;56(2):225-35. doi: 10.1038/icb.1978.24.
A study of immunoglobulin and albumin concentrations and the flow of intestinal lymph was made in sheep during infection with the parasitic nematode, Trichostrongylus colubriformis. Observations were made on uninfected worm-free sheep, on worm-free sheep undergoing primary infection and on immune sheep undergoing challenge infection. The flow of intestinal lymph and the concentrations of IgG1, IgG2 and IgM in intestinal lymph and IgG2 and IgM in blood plasma remained relatively constant during both primary and challenge infection. The concentration of IgA in intestinal lymph and blood plasma increased as early as day 6 of primary infection and maximum values were observed at day 34. By day 34 of primary infection the concentration of IgG1 in blood plasma was significantly greater than that found in worm-free controls. Plasma IgG1 concentrations in resistant sheep were also significantly greater than those observed in worm-free controls. Challenge infection in immune sheep did not raise the concentration of IgA in intestinal lymph and blood or IgG1 in blood. An indirect haemagglutination test was used to detect antibodies to T. colubriformis. Primary infection did not increase anti-worm antibody titres in either intestinal lymph or serum. Significant antibody titres were present in serum and lymph from immune sheep and were rapidly increased by challenge infection. Most antibody activity in serum was associated with IgM, whereas that in intestinal lymph was associated with IgA. These observations indicate the occurrence of a local antibody response to T. colubriformis in the intestine of immune sheep.
对感染寄生线虫——蛇形毛圆线虫的绵羊的免疫球蛋白和白蛋白浓度以及肠淋巴液流量进行了研究。观察对象包括未感染且无寄生虫的绵羊、处于初次感染的无寄生虫绵羊以及接受攻击感染的免疫绵羊。在初次感染和攻击感染期间，肠淋巴液流量以及肠淋巴液中IgG1、IgG2和IgM的浓度，还有血浆中IgG2和IgM的浓度都保持相对稳定。初次感染的第6天，肠淋巴液和血浆中IgA的浓度就开始升高，在第34天达到最大值。到初次感染的第34天，血浆中IgG1的浓度显著高于无寄生虫对照组。抗性绵羊的血浆IgG1浓度也显著高于无寄生虫对照组。免疫绵羊的攻击感染并未提高肠淋巴液和血液中IgA的浓度，也未提高血液中IgG1的浓度。采用间接血凝试验检测抗蛇形毛圆线虫抗体。初次感染并未增加肠淋巴液或血清中的抗虫抗体滴度。免疫绵羊的血清和淋巴液中存在显著的抗体滴度，且攻击感染使其迅速升高。血清中的大多数抗体活性与IgM相关，而肠淋巴液中的抗体活性与IgA相关。这些观察结果表明，免疫绵羊的肠道中出现了针对蛇形毛圆线虫的局部抗体反应。
