Halacková Z, Oudrán L, Kukletová M
Acta Histochem. 1980;67(2):173-9. doi: 10.1016/S0065-1281(80)80021-3.
The authors studied alkaline and acid phosphatase, nonspecific esterase and NADH2 tetrazolium reductase localization in the periodontium of the rat molar. The activity of all enzymes studied remained preserved in spite of decalcification in EDTA. The activity of the enzymes was relatively high, the most intense reaction was observed, with the exception of alkaline phosphatase, in the gingival epithelium. In the junctional epithelium the reaction was less intense and decreased towards the end of the epithelial attachment. Only NADH2 tetrazolium reductase exhibited high activity in this region. The periodontal ligament displayed positive reaction in all enzymes studied with the exception of alkaline phosphatase, which was active only in the dentoalveolar ligament. The results of the present study indicate that highly active metabolic processes take place in the periodontium. The results and literature are discussed in the paper.
作者研究了大鼠磨牙牙周组织中碱性磷酸酶、酸性磷酸酶、非特异性酯酶和NADH2四氮唑还原酶的定位。尽管用乙二胺四乙酸（EDTA）脱钙，但所研究的所有酶的活性仍得以保留。酶的活性相对较高，除碱性磷酸酶外，在牙龈上皮观察到最强烈的反应。在结合上皮中，反应较弱，且朝着上皮附着末端逐渐减弱。只有NADH2四氮唑还原酶在该区域表现出高活性。牙周膜对所研究的所有酶均呈阳性反应，但碱性磷酸酶仅在牙槽韧带中具有活性。本研究结果表明牙周组织中发生着高度活跃的代谢过程。本文对这些结果及相关文献进行了讨论。
