Pfister C, Wolny H J
Acta Histochem. 1980;67(2):195-9.
As a result of the reaction of ninhydrin with gamma-aminobutyric acid and glutamic acid in the octanolic milieu, a fluorescent product, presumably a copper-II-chelate-complex, was formed. Partial reaction steps were investigated spectrofluorometrically. The excitation and emmission spectra resulting from the reaction between ninhydrin and glutamic acid, ninhydrin and gamma-aminobutyric acid, and ninhydrin, glutamic acid and gamma-aminobutyric acid were measured. Possible consequences are discussed with respect to fluorescence histochemical procedures.
在辛醇环境中,茚三酮与γ-氨基丁酸和谷氨酸反应产生了一种荧光产物,推测为铜(II)螯合物复合物。通过荧光分光光度法研究了部分反应步骤。测量了茚三酮与谷氨酸、茚三酮与γ-氨基丁酸以及茚三酮、谷氨酸和γ-氨基丁酸之间反应产生的激发光谱和发射光谱。针对荧光组织化学程序讨论了可能的结果。