Jouanneau J, Fournet B, Bourrillon R
Biochim Biophys Acta. 1981 Feb 27;667(2):277-84. doi: 10.1016/0005-2795(81)90193-8.
The structure of a mannose-rich glycopeptide from a human pathological IgM has been investigated. It belongs to the group I (simple) glycopeptides and contains only mannose and N-acetylglucosamine residues in a molar ratio of 10:2. The structures of its oligosaccharide moiety and peptide chain have been determined: its molecular localization is specified and the relation between its biosynthesis and the oligosaccharide structure determine is discussed. Based on the alpha- and beta-mannosidase digestions and permethylation studies for the oligosaccharide moiety, and on the results obtained after sequential analysis of the peptide chain, the following structure is proposed for the mannose-rich IgM Du glycopeptide: (Formula: see text). The recovery of one molecule of this glycopeptide per molecule of heavy chain and the determination of the amino acid sequence have led us to locate this glycopeptide on asparagine 402 of the Fc portion of the heavy chain mu of IgM Du.
对来自人类病理性IgM的富含甘露糖的糖肽结构进行了研究。它属于I组（简单）糖肽，仅含有甘露糖和N-乙酰葡糖胺残基，摩尔比为10:2。已确定其寡糖部分和肽链的结构：明确了其分子定位，并讨论了其生物合成与确定的寡糖结构之间的关系。基于对寡糖部分的α-和β-甘露糖苷酶消化及全甲基化研究，以及肽链序列分析后获得的结果，提出了富含甘露糖的IgM Du糖肽的以下结构：（公式：见正文）。每条重链分子中该糖肽分子的回收率以及氨基酸序列的确定，使我们将该糖肽定位在IgM Du重链μ的Fc部分的天冬酰胺402上。
