Wünsch E, Wendlberger G, Mladenova-Orlinova L, Göhring W, Jaeger E, Scharf R
Hoppe Seylers Z Physiol Chem. 1981 Feb;362(2):179-83.
The synthesis of the tetratriacontapeptide amide corresponding to the revised structure of human big gastrin I is described. The fully protected peptide derivative was obtained by assembly in sequence order of the suitably protected fragments [1--9], [10--14] and [15--34] via the dicyclohexylcarbodiimide/N-hydroxysuccinimide and azide method, respectively. Upon removal of the protecting groups by exposure to trifluoroacetic acid and purification of the resulting crude product by chromatographic methods, human big gastrin I was obtained in satisfactory yields and at a high degree of purity. The identical immunological crossreactivities of natural and synthetic human big gastrin I using anti-porcine big gastrin I antiserum strongly supports the correctness of the newly proposed primary structure of this member of the gastrin family.
本文描述了与人类大胃泌素I修正结构相对应的三十四肽酰胺的合成。通过分别采用二环己基碳二亚胺/ N - 羟基琥珀酰亚胺法和叠氮法，按顺序组装适当保护的片段[1 - 9]、[10 - 14]和[15 - 34]，得到了完全保护的肽衍生物。通过用三氟乙酸脱保护基团，并通过色谱方法纯化所得粗产物，以令人满意的产率和高纯度获得了人类大胃泌素I。使用抗猪大胃泌素I抗血清时，天然和合成的人类大胃泌素I具有相同的免疫交叉反应性，这有力地支持了胃泌素家族这一成员新提出的一级结构的正确性。
