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产孢微生物中的β-1,3-1,4-葡聚糖酶。IV. 某些芽孢杆菌β-葡聚糖水解酶的性质(作者译)

[beta-1,3-1,4-glucanase in spore-forming microorganisms. IV. Properties of some Bacillus-beta-glucan-hydrolases (author's transl)].

作者信息

Borriss R, Zemek J

出版信息

Zentralbl Bakteriol Naturwiss. 1981;136(1):63-9.

PMID:6784378
Abstract

The beta-glucan-hydrolases produced by seven Bacillus species have been characterized with regard to some physicochemical properties. Ca-ions stabilize all tested glucanases. Optimum pH and optimum temperature were found to be different for the investigated enzymes. In the work presented here is given further characteristics of Bacillus-beta-glucan-hydrolases as pH-stability and temperature stability, sensibility on glucono-1,5-lactone, as well known inhibitor of carbohydrases, and electrophoretic mobility on cellulose acetate-sheets.

摘要

已对七种芽孢杆菌属细菌产生的β-葡聚糖水解酶的一些物理化学性质进行了表征。钙离子可稳定所有测试的葡聚糖酶。研究发现,所研究的酶的最适pH值和最适温度各不相同。本文进一步阐述了芽孢杆菌β-葡聚糖水解酶的其他特性,如pH稳定性、温度稳定性、对糖酶的已知抑制剂葡萄糖酸-1,5-内酯的敏感性以及在醋酸纤维素薄膜上的电泳迁移率。

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