Baskerville A, Cox S
Br J Exp Pathol. 1978 Jun;59(3):327-31.
Mice were injected with up to 9 doses of methotrexate and killed at intervals after treatment. Liver changes were assessed histopathologically and by image analysis, and included vacuolation and ballooning degeneration of centrilobular liver cells. There was considerable irregularity in nuclear size in methotrexate-treated mice, in which hepatic nuclei were significantly larger than those of control animals. Fatty change was not induced in the liver by these doses of methotrexate, and there was no cellular infiltration or fibrosis.
给小鼠注射多达9剂甲氨蝶呤，并在治疗后的不同时间点处死。通过组织病理学和图像分析评估肝脏变化，包括中央小叶肝细胞的空泡化和气球样变性。甲氨蝶呤治疗的小鼠细胞核大小存在相当大的不规则性，其中肝细胞核明显大于对照动物的肝细胞核。这些剂量的甲氨蝶呤未在肝脏中诱导脂肪变性，也没有细胞浸润或纤维化。
