Bergfeld J, Fliess F R
Arch Exp Veterinarmed. 1981;35(1):143-8.
The doses of FSH (follicle-stimulating hormone), PMSG (pregnant mare serum gonadotrophin), and gn-RH (gonadotrophin-releasing hormone) effective in terms of triggering ovulation were determined in a mouse ovulation test. Varying doses of the above preparations were subcutaneously injected, 48 hours after overstimulation by injection of 0.5 or 1.0 IU of PMSG. The animals were sacrificed for examination, after another 18-20 hours had passed. Roughly 50 per cent of all animals treated (threshold) in one and the same dosage group (n = 5) had ovulated in response to 0.02-0.1 IU of FSH per animal. The following FSH and PMSG dosages are recommended: 0.02, 0.04, 0.06, 0.08, and 0.1 IU of FSH, 0.6, 1.0, 1.4, 1.8, 2.2, 2.6, 3.0 IU of PMSG. When mouse ovulation tests were used in orientation studies, ovulation was regularly induced by Gn-RH doses per animal between 0.01 and 1.0 micrograms. Dosage spacings or increments should be specified with higher accuracy by further studies.
通过小鼠排卵试验确定了促卵泡激素（FSH）、孕马血清促性腺激素（PMSG）和促性腺激素释放激素（gn-RH）在触发排卵方面的有效剂量。在注射0.5或1.0国际单位PMSG进行超刺激48小时后，皮下注射不同剂量的上述制剂。再过18 - 20小时后，处死动物进行检查。在同一剂量组（n = 5）中，每只动物注射0.02 - 0.1国际单位FSH后，约50%接受治疗的动物（阈值）排卵。建议使用以下FSH和PMSG剂量：0.02、0.04、0.06、0.08和0.1国际单位FSH，0.6、1.0、1.4、1.8、2.2、2.6、3.0国际单位PMSG。在定向研究中使用小鼠排卵试验时，每只动物注射0.01至1.0微克Gn-RH可定期诱导排卵。进一步的研究应更精确地确定剂量间隔或增量。
