Loor R, Shimano T, Manzo M L, van Dusen L, Papsidero L D, Nicolai J J, Tytgat G N, Chu T M
Biochim Biophys Acta. 1981 Apr 28;668(2):222-34. doi: 10.1016/0005-2795(81)90030-1.
A pancreas-specific antigen was identified by immunologic techniques and purified from saline extract of human pancreas. The purified pancreas-specific antigen was shown to be homogeneous by polyacrylamide gel electrophoresis under both denaturing and non-denaturing conditions. It had a molecular weight of 44000 as estimated by gel filtration or sodium dodecyl sulfate-gel electrophoresis, and a sedimentation coefficient of 3.4 S as analyzed by sucrose gradient centrifugation. Pancreas-specific antigen possessed an isoelectric point of 4.9 and migrated to alpha-beta region upon immunoelectrophoresis. By colorimetric assay procedures, pancreas-specific antigen exhibited no enzyme activity, such as amylase, protease, esterase, lipase, acid phosphatase, alkaline phosphatase peroxidase, deoxyribonuclease or ribonuclease. Immunoreactivity of pancreas-specific antigen was sensitive to proteolytic enzymes, perchloric acid and high temperature (70 degrees C, 10 min); but insensitive to neuraminidase or beta-glucosidase. Immunohistochemical staining revealed that pancreas-specific antigen was located in acinar cells of human pancreas. In addition, a higher concentration of pancreas-specific antigen was detected in pancreatic juice than in the saline extract of pancreas. This newly identified pancreas-specific antigen, therefore, may be a useful marker protein in physiological studies of pancreas and pancreatic secretion.
一种胰腺特异性抗原通过免疫技术被鉴定出来，并从人胰腺的盐水提取物中纯化得到。在变性和非变性条件下，通过聚丙烯酰胺凝胶电泳显示纯化的胰腺特异性抗原是均一的。通过凝胶过滤或十二烷基硫酸钠-凝胶电泳估计其分子量为44000，通过蔗糖梯度离心分析其沉降系数为3.4S。胰腺特异性抗原的等电点为4.9，在免疫电泳时迁移至α-β区。通过比色测定法，胰腺特异性抗原未表现出淀粉酶、蛋白酶、酯酶、脂肪酶、酸性磷酸酶、碱性磷酸酶、过氧化物酶、脱氧核糖核酸酶或核糖核酸酶等酶活性。胰腺特异性抗原的免疫反应性对蛋白水解酶、高氯酸和高温（70℃，10分钟）敏感；但对神经氨酸酶或β-葡萄糖苷酶不敏感。免疫组织化学染色显示胰腺特异性抗原位于人胰腺的腺泡细胞中。此外，在胰液中检测到的胰腺特异性抗原浓度高于胰腺的盐水提取物。因此，这种新鉴定的胰腺特异性抗原可能是胰腺和胰腺分泌生理研究中的一种有用的标记蛋白。
