Prince D A, Wong R K
Brain Res. 1981 Apr 6;210(1-2):323-33. doi: 10.1016/0006-8993(81)90905-7.
The in vitro neocortical brain slice technique was used to study electrophysiological properties of neurons from brain biopsies in 10 patients undergoing neurosurgical treatment for a variety of conditions, including focal epilepsy. The principal finding was the occurrence of orthodromically evoked depolarization shifts (DSs) and burst discharges in a proportion of neurons in slices from epileptogenic cortex. These evoked depolarizations and bursts had a number of properties in common with those from experimental epileptogenic foci in neocortex, including large amplitude and prolonged duration; long and variable latencies; and all or none, threshold type behavior, dependent on the parameters of orthodromic stimulation. Also DSs could not be evoked by intracellular stimulation, or blocked by hyperpolarizing current pulses once they had been orthodromically evoked. Responses of DSs to current thus differed markedly from those of neurons in epileptogenic guinea pig hippocampal slices. The results of these experiments suggest that intracellular events in human neurons involved in epileptogenesis are similar in appearance to those in various animal models. Neurons in chronic epileptogenesis are similar in appearance to those in various animal models. Neurons in chronic epileptogenic foci retain some of their abnormal properties within brain slices maintained in vitro.
采用体外新皮质脑片技术，研究了10例因各种病症（包括局灶性癫痫）接受神经外科治疗患者脑活检神经元的电生理特性。主要发现是，在来自致痫皮质的脑片的一部分神经元中出现了顺向诱发去极化偏移（DSs）和爆发性放电。这些诱发的去极化和爆发与新皮质实验性致痫灶的去极化和爆发有许多共同特性，包括幅度大、持续时间长；潜伏期长且可变；以及全或无的阈值型行为，这取决于顺向刺激的参数。此外，DSs不能通过细胞内刺激诱发，一旦被顺向诱发，也不能被超极化电流脉冲阻断。因此，DSs对电流的反应与致痫豚鼠海马脑片神经元的反应明显不同。这些实验结果表明，参与癫痫发生的人类神经元的细胞内事件在外观上与各种动物模型中的相似。慢性癫痫发生中的神经元在外观上与各种动物模型中的相似。慢性致痫灶中的神经元在体外维持的脑片中保留了一些异常特性。
