Schwartzkroin P A, Turner D A, Knowles W D, Wyler A R
Ann Neurol. 1983 Mar;13(3):249-57. doi: 10.1002/ana.410130305.
The in vitro slice technique was used to study neuronal activity in human cortical tissue removed during neurosurgical procedures for intractable epilepsy and in monkey neocortex rendered epileptogenic by injection of alumina gel. In both cases, biopsies were guided by electrocorticographic signs of epileptiform activity. Intracellular recordings were made from 167 neurons in human tissue and from 73 neurons in monkey tissue samples. There was little spontaneous activity in these biopsies and no indication of spontaneous cellular bursting. Stimulation at the pial surface or in white matter evoked synaptically driven activity that was primarily excitatory. Graded bursts of activity could be elicited from some cells, but no all-or-none paroxysmal depolarization shifts were recorded. Inhibitory postsynaptic potentials were relatively rare. Intracellular injections of dye in a small number of neurons revealed no obvious differences between bursting and nonbursting neurons. These in vitro studies of chronic epileptic cortex have thus far provided few clear insights into the basic mechanisms of epilepsy.
体外切片技术被用于研究在难治性癫痫神经外科手术过程中切除的人类皮质组织以及通过注射氧化铝凝胶而致痫的猴新皮质中的神经元活动。在这两种情况下，活检均由癫痫样活动的皮质电图征象引导。对人类组织中的167个神经元和猴组织样本中的73个神经元进行了细胞内记录。这些活检组织中几乎没有自发活动，也没有自发细胞爆发的迹象。软脑膜表面或白质的刺激诱发了主要为兴奋性的突触驱动活动。一些细胞可引发分级活动爆发，但未记录到全或无的阵发性去极化偏移。抑制性突触后电位相对少见。对少数神经元进行细胞内染料注射显示，爆发性神经元和非爆发性神经元之间没有明显差异。迄今为止，这些对慢性癫痫皮质的体外研究几乎没有为癫痫的基本机制提供明确的见解。
