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一种测定DNA分子量分布的简单方法。

A simple method for the determination of DNA molecular weight distribution.

作者信息

Surowiec A

出版信息

J Biochem Biophys Methods. 1981 Feb;4(2):91-100. doi: 10.1016/0165-022x(81)90022-1.

DOI:10.1016/0165-022x(81)90022-1
PMID:6785340
Abstract

A method is described for the molecular weight distribution of DNA which is determined from sedimentation-velocity analysis. Knowing the distribution of sedimentation coefficients for a single DNA concentration it is possible to extrapolate such a distribution to infinite dilution of the solute in a simple way. Two versions (using two or three terms of a series) of extrapolating equations are considered and discussed in detail. The sedimentation coefficients distribution calculated from these equations differs only insignificantly with that obtained in a conventional way.

摘要

本文描述了一种根据沉降速度分析来测定DNA分子量分布的方法。已知单一DNA浓度下的沉降系数分布，就可以用一种简单的方法将这种分布外推到溶质无限稀释的情况。文中考虑并详细讨论了两种外推方程的版本（使用级数的两项或三项）。由这些方程计算得到的沉降系数分布与用传统方法得到的结果仅有微小差异。

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