Mingot F, Jorcano J L, Acuña M I, Davila C A
Biochim Biophys Acta. 1976 Feb 5;418(3):315-20. doi: 10.1016/0005-2787(76)90293-8.
Evidence is presented of the constancy in the conformation of denatured DNA in 2% formaldehyde in SSC (0.15 M NaCl/0.015 M trisodium citrate (pH 7.0)) over a wide range of molecular weights. It is also shown that denatured RNA behaves in the same way as DNA. The range of molecular weights studied runs from 0.02 to 16 X 10(6). In accordance with these results, biparametric expressions are proposed for molecular weight calculations from sedimentation or viscosity data of denatured DNA or RNA, when determined in 2% formaldehyde in SSC. Testing of the expressions with standard DNA and RNA preparations showed good correlation.
有证据表明，在一系列很宽的分子量范围内，变性DNA在含2%甲醛的SSC（0.15M氯化钠/0.015M柠檬酸三钠（pH7.0））中的构象具有稳定性。还表明变性RNA的行为与DNA相同。所研究的分子量范围从0.02到16×10⁶。根据这些结果，当在含2%甲醛的SSC中测定变性DNA或RNA的沉降或粘度数据时，提出了用于计算分子量的双参数表达式。用标准DNA和RNA制剂对这些表达式进行测试显示出良好的相关性。
