Hommes F A, Everts R S
Biol Neonate. 1978;33(3-4):193-200. doi: 10.1159/000241072.
The association of hexokinase with particulate fractions of liver has been studied during the development of the rat. In the fetal rat liver about 50% of the total hexokinase is bound to the mitochondria. This proportion decreases gradually after birth. No association of hexokinase with mitochondria could be demonstrated in the adult rat liver. It is suggested that the binding of hexokinase to the mitochondria is a consequence of a property which is lost during development of the mitochondrial membranes. At least part of the hexokinase is rather tightly bound to the mitochondria. Glucose-6-phosphate or ATP do not solubilize the enzyme. The proportion of hexokinase bound to the mitochondria is independent of the metabolic state of the hepatocyte as exemplified by studies with inhibitors of oxidative phosphorylation, using the digitonin technique for the separation of particulate and cytosol fractions of isolated fetal rat hepatocytes.
在大鼠发育过程中，对己糖激酶与肝脏颗粒部分的关联进行了研究。在胎鼠肝脏中，约50%的总己糖激酶与线粒体结合。出生后这一比例逐渐降低。在成年大鼠肝脏中未证实己糖激酶与线粒体有联系。有人提出，己糖激酶与线粒体的结合是线粒体膜发育过程中丧失的一种特性的结果。至少部分己糖激酶与线粒体结合相当紧密。葡萄糖-6-磷酸或ATP不能使该酶溶解。结合到线粒体上的己糖激酶比例与肝细胞的代谢状态无关，以使用洋地黄皂苷技术分离离体胎鼠肝细胞的颗粒和胞质部分，并以氧化磷酸化抑制剂进行研究为例。
