Marold L M, Freedman R, Chamberlain R E, Miyashiro J J
Appl Environ Microbiol. 1981 Apr;41(4):977-80. doi: 10.1128/aem.41.4.977-980.1981.
Results of minimal inhibitory concentration tests with a diversity of bacterial strains showed that 9-chloro-9-(4-diethylaminophenyl)-10-phenylacridan (C-390) inhibited the growth of all microorganisms tested (other than Pseudomonas aeruginosa) at 25 microgram/ml or less, whereas MICs obtained for P. aeruginosa ranged from to to greater than 100 microgram/ml. Therefore, C-390 was evaluated as a potential selective agent for P. aeruginosa in pseudomonas agar F. Recovery tests were conducted on this medium with 53 strains o P. aeruginosa, and the results were compared to those obtained in similar tests on commercially available selective media, i.e., pseudomonas isolation agar and Pseudosel agar. The results of these comparisons indicated that pseudomonas agar F with C-390 was significantly less inhibitory than Pseudosel agar and pseudomonas isolation agar and more selective than pseudomonas isolation agar. The incorporation of C-390 in pseudomonas agar F also provided a medium that was both selective and differential. Preliminary evidence also suggested that C-390 may be added to other basal media with comparable results.
对多种细菌菌株进行的最低抑菌浓度测试结果表明，9-氯-9-(4-二乙氨基苯基)-10-苯基吖啶（C-390）在25微克/毫升或更低浓度时可抑制所有测试微生物（铜绿假单胞菌除外）的生长，而铜绿假单胞菌的最低抑菌浓度范围为100微克/毫升至高于100微克/毫升。因此，在假单胞菌琼脂F中，C-390被评估为铜绿假单胞菌的一种潜在选择剂。对53株铜绿假单胞菌在该培养基上进行了回收率测试，并将结果与在市售选择培养基（即假单胞菌分离琼脂和假单胞菌选择琼脂）上进行的类似测试结果进行了比较。这些比较结果表明，添加C-390的假单胞菌琼脂F的抑制性明显低于假单胞菌选择琼脂和假单胞菌分离琼脂，且比假单胞菌分离琼脂更具选择性。在假单胞菌琼脂F中加入C-390还提供了一种兼具选择性和鉴别性的培养基。初步证据还表明，C-390可添加到其他基础培养基中，结果相当。
