de Klerk G, Kruiswijk C, Hart A A, Goudsmit R
Blood. 1978 Sep;52(3):560-8.
Investigations on the mouse fetal liver cell bioassay for erythropoietin (ESF) have revealed that iron present in test sera significantly dilutes the radiolabel (59Fe) and thus decreases 59Fe incorporation into heme. A method of correction for the influence of iron on the dose-response relationship of human sera is presented. Application of this method made it possible to assay human sera up to culture concentrations of 150 microliter/ml. It was shown that a corrected serum dose-response curve showed parallelism to the curve of an ESF standard preparation. This suggests similarity of the active principles and allows valid estimation of a potency ratio.
对用于促红细胞生成素（ESF）的小鼠胎儿肝细胞生物测定法的研究表明，测试血清中存在的铁会显著稀释放射性标记物（59Fe），从而减少59Fe掺入血红素中。本文提出了一种校正铁对人血清剂量反应关系影响的方法。应用该方法能够测定培养浓度高达150微升/毫升的人血清。结果表明，校正后的血清剂量反应曲线与ESF标准制剂的曲线平行。这表明活性成分相似，并允许有效估计效价比。
