de Klerk G, Hart A A, Kruiswijk C, Goudsmit R
Blood. 1978 Sep;52(3):569-77.
A modification of the mouse fetal liver cell bioassay for erythropoietin (ESF) that allows quantitative detection of ESF in human serum is described. The modification consists of (1) correction for the effect of serum iron on 59Fe incorporation into heme and (2) the use of an "internal standard," i.e., a standard ESF preparation dissolved in the assayed test serum. As a result of this modification the statistical method of bioassay analysis had to be changed fundamentally. The mean serum concentration of ESF measured in 20 normal males was 48 microunit/ml, as compared to 29 microunit/ml in 18 females. The difference was significant at p = 0.017. The stimulatory activity of a human serum on 59Fe incorporation into heme could be neutralized by an anti-human ESF immune serum.
本文描述了一种用于促红细胞生成素（ESF）的小鼠胎儿肝细胞生物测定法的改进方法，该方法可对人血清中的ESF进行定量检测。改进内容包括：（1）校正血清铁对59Fe掺入血红素的影响；（2）使用“内标”，即将标准ESF制剂溶解在所检测的试验血清中。由于这一改进，生物测定分析的统计方法必须进行根本性改变。在20名正常男性中测得的ESF平均血清浓度为48微单位/毫升，而18名女性中的浓度为29微单位/毫升。差异在p = 0.017时具有显著性。人血清对59Fe掺入血红素的刺激活性可被抗人ESF免疫血清中和。
