Kavanagh M L, Wood C N, Davidson J F
Thromb Haemost. 1981 Feb 23;45(1):60-4.
Nine human antibodies to factor VIII were isolated from haemophilic plasmas by affinity chromatography and gel filtration and six were subsequently subjected to immunological characterization. Three partially purified preparations were similarly characterized. Eight of the antibodies were characterized as being exclusively IgG and one preparation was found to contain IgM. Seven of the antibodies contained only a single light chain type, four being of type lambda and three of type kappa. Two antibody preparations contained both kappa and lambda light chains. In four of the preparations, only a single heavy chain sub-class could be demonstrated, three of IgG3 and one of IgG4. Of the remainder, three were a mixture of IgG3 and IgG4 sub-classes and one contained both IgG2 and IgG4. IgG sub-classification could not be achieved with the IgM-containing preparation. These results demonstrate a restricted heterogeneity of light and heavy chains in human antibodies to factor VIII.
通过亲和层析和凝胶过滤从血友病患者血浆中分离出九种抗凝血因子VIII的人源抗体，随后对其中六种进行了免疫学特性鉴定。对三种部分纯化的制剂也进行了类似的特性鉴定。其中八种抗体被鉴定为仅为IgG，一种制剂被发现含有IgM。七种抗体仅包含单一轻链类型，四种为λ型，三种为κ型。两种抗体制剂同时含有κ链和λ链。在四种制剂中，仅能鉴定出单一重链亚类，三种为IgG3，一种为IgG4。其余的制剂中，三种是IgG3和IgG4亚类的混合物，一种同时含有IgG2和IgG4。含IgM的制剂无法进行IgG亚类分类。这些结果表明，人抗凝血因子VIII抗体的轻链和重链具有有限的异质性。
