风疹IgG抗体亚类及轻链类型的鉴定
Characterization of the subclasses and light chain types of IgG antibodies to rubella.
作者信息
Skvaril F, Schilt U
出版信息
Clin Exp Immunol. 1984 Mar;55(3):671-6.
Abstract
IgG subclasses of antibodies to rubella were determined in indirect enzyme linked immunoassay (ELISA) with monoclonal antibodies specific for human IgG1, IgG2, IgG3 and IgG4. Eleven sera from women with long past history of rubella, two hyperimmune and five non-hyperimmune immunoglobulin preparations were tested. Light chain types of the antibodies were tested in ELISA with polyclonal specific antibodies to kappa and lambda chains. Antibodies to rubella in the sera as well as in the immunoglobulin preparations were found in the IgG1 subclass only. Both light chain types were present in the antibodies.
摘要
采用针对人IgG1、IgG2、IgG3和IgG4的单克隆抗体，通过间接酶联免疫吸附测定（ELISA）法测定风疹抗体的IgG亚类。检测了11名有风疹病史的女性的血清、2种高效免疫球蛋白制剂和5种非高效免疫球蛋白制剂。采用针对κ链和λ链的多克隆特异性抗体，通过ELISA法检测抗体的轻链类型。血清及免疫球蛋白制剂中的风疹抗体仅存在于IgG1亚类中。两种轻链类型均存在于抗体中。
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