Kozlov A V, Dmitrieva E Iu
Biokhimiia. 1980 Sep;45(9):1654-62.
A "heavy" nucleoid (folded chromosome) from A. variabilis has been isolated in preparative amounts. The composition of the folded chromosome and that of a more simple DNA--protein complex isolated from the "heavy" nucleoid of A. variabilis by chromatography on a column with methylated albumin (MAK) were studied. It was shown that the "heavy" nucleoids contain total cell DNA in a complex with the definite membrane fragment, which can be discovered by a large number of membrane proteins, phospholipids, lipopolysaccharides and amino sugars. After MAK chromatography the DNA--protein complex also contains total cellular DNA, a negligible amount of membrane polypeptides and noticeable amounts of phospholipids and lipopolysaccharides.
已制备出大量来自可变鱼腥藻的“重”类核(折叠染色体)。对折叠染色体以及通过在甲基化白蛋白(MAK)柱上进行色谱分离从可变鱼腥藻“重”类核中分离出的更简单的DNA - 蛋白质复合物的组成进行了研究。结果表明,“重”类核包含与特定膜片段结合的总细胞DNA,该膜片段可通过大量膜蛋白、磷脂、脂多糖和氨基糖被发现。经过MAK色谱分离后,DNA - 蛋白质复合物也包含总细胞DNA、少量的膜多肽以及显著量的磷脂和脂多糖。