Chetina E V, Suzina N E, Trotsenko Iu A
Biokhimiia. 1981 Nov;46(11):2100-9.
The cell wall (CW), cytoplasmic (CM) and intracytoplasmic membrane (ICM) fractions were obtained by sucrose linear gradient centrifugation of membrane preparations of Methylomonas methanica. The CW fraction represented by large open fragments with 5-layer structure is enriched in lipopolysaccharides and is similar in its protein composition to the CW fractions of other gram-negative bacteria. The CM vesicles are surrounded with a unit membrane, have high activities of marker enzymes (NADH dehydrogenase, succinate dehydrogenase and ATPase), a higher phospholipid content, cytochromes of a-, b- and c-types and differ qualitatively in its protein composition from the CW fraction. The ICM fraction has much in common with the CM fraction but differs from it by a higher level of marker enzymes and cytochromes of b and c types.
通过对甲基单胞菌甲烷氧化菌的膜制剂进行蔗糖线性梯度离心，获得了细胞壁（CW）、细胞质（CM）和胞内膜（ICM）组分。以具有5层结构的大的开放片段为代表的CW组分富含脂多糖，其蛋白质组成与其他革兰氏阴性菌的CW组分相似。CM囊泡被单位膜包围，具有较高的标记酶（NADH脱氢酶、琥珀酸脱氢酶和ATP酶）活性、较高的磷脂含量、a-、b-和c-型细胞色素，并且其蛋白质组成在质量上与CW组分不同。ICM组分与CM组分有许多共同之处，但在b型和c型标记酶和细胞色素水平上与之不同。
