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Immunochemical visualization of binding of the chemical carcinogen benzo(a)pyrene diol-epoxide 1 to the genome.

作者信息

Slor H, Mizusawa H, Neihart N, Kakefuda T, Day R S, Bustin M

出版信息

Cancer Res. 1981 Aug;41(8):3111-7.

PMID:6788367
Abstract

antisera against DNA modified with r-7,t-8-dihydroxy-9,10-oxy-7,8,9,10-tetrahydrobenzo(a)pyrene (BPDE-1) was elicited in rabbits. Such sera reached with either single- or double-stranded modified DNA but not with unmodified DNA, free benzo(a)pyrene, or proteins modified by BPDE-1. Indirect immunofluorescence studies indicated that the immunoglobulin G in the sera bound specifically to the nuclei of KD cells which were treated with BPDE-1. The intensity of fluorescence was proportional to the dose of BPDE-1 used to treat the cells. About 50% of the BPDE-1-DNA adducts remained bound to DNA 24 hr after the removal of the carcinogen. The location of BPDE-1-modified bases in Col E1 DNA was visualized by immunoelectron microscopy.

摘要

相似文献

1
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引用本文的文献

1
Site-specific carcinogen binding to DNA in polytene chromosomes.位点特异性致癌物与多线染色体中DNA的结合。
Proc Natl Acad Sci U S A. 1985 Oct;82(20):7076-80. doi: 10.1073/pnas.82.20.7076.
2
Use of monoclonal and polyclonal antibodies against DNA adducts for the detection of DNA lesions in isolated DNA and in single cells.使用针对DNA加合物的单克隆抗体和多克隆抗体来检测分离的DNA和单细胞中的DNA损伤。
Environ Health Perspect. 1985 Oct;62:81-8. doi: 10.1289/ehp.856281.
3
Polyclonal antibodies to DNA modified with 4-nitroquinoline 1-oxide: application for the detection of 4-nitroquinoline 1-oxide-DNA adducts in vivo.
针对经4-硝基喹啉1-氧化物修饰的DNA的多克隆抗体：在体内检测4-硝基喹啉1-氧化物-DNA加合物的应用。
Jpn J Cancer Res. 1988 Feb;79(2):195-203. doi: 10.1111/j.1349-7006.1988.tb01577.x.