Toutant M, Toutant J P, Renaud D, Le Douarin G, Changeux J P
C R Seances Acad Sci III. 1981 Mar 23;292(12):771-5.
From incubation day 10 to 15, the spinal cord of Chick embryos are electrically stimulated in ovo at 0.5 Hz at the level of the motor roots innervating the latissimus dorsi muscles. As a consequence, the total number of spots of acetylcholinesterase determined on serial sections of posterior latissimus dorsi muscle increased 2.3 fold. This increase parallels that of acetylcholine receptor clusters, supporting the interpretation that these clusters are of synaptic origin.
从孵化第10天到15天，在鸡胚脊髓中，于支配背阔肌的运动神经根水平以0.5赫兹的频率进行卵内电刺激。结果，在背阔肌后部连续切片上测定的乙酰胆碱酯酶斑点总数增加了2.3倍。这种增加与乙酰胆碱受体簇的增加平行，支持了这些簇起源于突触的解释。
