Bourgeois J P, Toutant M
Reprod Nutr Dev (1980). 1982;22(1B):235-41.
The effects of a reduction or an increase in muscular activity during embryonic life were investigated in the posterior latissimus dorsi muscle of the chick embryo. The biochemical differentiation, number and localization of the developing neuromuscular junctions were studied after curarization or chronic medullar stimulation. The degradation of junctional as well as extrajunctional PLD cholinergic receptor sites was constant (about 28 h) up to day 10 after hatching, and therefore the disappearance of extrajunctional ACh-R at the end of embryonic life was due to a shut-off of their synthesis. Curarization did not modify the focal innervation of PLD fibres, whereas both the density of extrajunctional ACh-R and neurites per fibre increased. On the other hand, chronic stimulation led to the formation of supernumerary ACh-R clusters of PLD fibres with a parallel increase in the total number of spots of acetylcholinesterase activity per muscle.
在鸡胚背阔肌中研究了胚胎期肌肉活动减少或增加的影响。在箭毒化或慢性延髓刺激后，研究了发育中的神经肌肉接头的生化分化、数量和定位。孵化后至第10天，接头及接头外PLD胆碱能受体位点的降解是恒定的（约28小时），因此胚胎期结束时接头外ACh-R的消失是由于其合成停止。箭毒化并未改变PLD纤维的局部神经支配，而每根纤维的接头外ACh-R密度和神经突均增加。另一方面，慢性刺激导致PLD纤维形成多余的ACh-R簇，同时每块肌肉中乙酰胆碱酯酶活性斑点总数平行增加。
