溴化二甲基二十八烷基铵对小鼠腹腔巨噬细胞吞噬和消化单核细胞增生李斯特菌的影响。
Effects of dimethyldioctadecylammonium bromide on phagocytosis and digestion of Listeria monocytogenes by mouse peritoneal macrophages.
作者信息
Hofhuis F M, Van der Meer C, Kersten M C, Rutten V P, Willers J M
出版信息
Immunology. 1981 Jul;43(3):425-31.
Listeria monocytogenes was labelled with [3H]-thymidine and phagocytosis in vivo, measured after the intraperitoneal injection of killed or viable listeria. The loss of intracellular radioactivity after incubation in vitro was used as a measure for digestion. Killing was assessed by counting the numbers of viable listeria before and after in vitro incubation. Peritoneal macrophages from mice immunized with viable listeria showed better phagocytosis and digestion of both killed and viable listeria than macrophages from normal mice. Viable listeria were digested to a lesser degree than killed ones by both normal and immune macrophages. The simultaneous injection of the surfactant dimethyldioctadecylammonium bromide (DDA) with killed or viable listeria greatly depressed the digestion of listeria. Phagocytosis of viable listeria was not affected whereas that of killed listeria was slightly enhanced. The injection of 10(8) killed listeria mixed with DDA greatly impaired the digestion of viable listeria 7 days but not 1 day after injection. The impairment of digestion was accompanied by an increase in the number of intracellular viable listeria.
用[³H] - 胸腺嘧啶核苷标记单核细胞增生李斯特菌，并在腹腔注射灭活或活的李斯特菌后测定其体内吞噬作用。体外孵育后细胞内放射性的损失用作消化的指标。通过计算体外孵育前后活的李斯特菌数量来评估杀菌情况。用活的李斯特菌免疫的小鼠的腹腔巨噬细胞比正常小鼠的巨噬细胞对灭活和活的李斯特菌都表现出更好的吞噬和消化能力。正常和免疫巨噬细胞对活的李斯特菌的消化程度均低于对灭活李斯特菌的消化程度。将表面活性剂二甲基二辛基溴化铵（DDA）与灭活或活的李斯特菌同时注射，会大大抑制李斯特菌的消化。活的李斯特菌的吞噬作用不受影响，而灭活李斯特菌的吞噬作用略有增强。注射10⁸个与DDA混合的灭活李斯特菌，在注射7天后但不是1天后，会极大地损害活的李斯特菌的消化。消化受损伴随着细胞内活的李斯特菌数量增加。
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