Mort J S, Poole A R, Decker R S
J Histochem Cytochem. 1981 May;29(5):649-57. doi: 10.1177/29.5.6788835.
The proteinase cathepsin B has been localized in human fibroblasts using a monospecific antiserum that reacts in a precipitating manner with the denatured enzyme only. The distribution of staining as revealed by both direct and indirect immunofluorescence indicates a typical lysosomal localization similar to that observed for cathepsin D. Using dual localization methods employing fluorescein- and tetramethylrhodamine-labeled affinity purified antibodies both proteinases were usually observed in the same lysosome, indicating a combined role in intracellular digestion.
利用一种仅与变性酶发生沉淀反应的单特异性抗血清，已将蛋白酶组织蛋白酶B定位在人成纤维细胞中。直接和间接免疫荧光显示的染色分布表明其具有典型的溶酶体定位，类似于组织蛋白酶D的定位。使用荧光素和四甲基罗丹明标记的亲和纯化抗体的双重定位方法，通常在同一溶酶体中观察到这两种蛋白酶，表明它们在细胞内消化中具有联合作用。
