标记抗体的纯化与特异性控制（作者译）
[Purification and specificity control of labelled antibodies (author's transl)].
作者信息
Franz H, Mohr J
出版信息
Acta Histochem Suppl. 1980;22:41-6.
PMID:6789395
Abstract
A review is given about causes of unspecific binding and the possibility of its prevention. Unspecific binding can be caused by: binding of proteins and glycoproteins on cells or tissues by non-covalent links, undesirable antigen-antibody-reactions, non-immune affinity reactions, changes of the charge of antibodies depending on the labelling procedure. A method for fractionation of labelled antibodies by using precipitation with protamine sulphate is described.
摘要
本文综述了非特异性结合的原因及其预防的可能性。非特异性结合可能由以下原因引起：蛋白质和糖蛋白通过非共价键与细胞或组织结合、不良的抗原-抗体反应、非免疫亲和反应、抗体电荷因标记程序而发生变化。本文还描述了一种使用硫酸鱼精蛋白沉淀法对标记抗体进行分级分离的方法。
相似文献
1
[Purification and specificity control of labelled antibodies (author's transl)].标记抗体的纯化与特异性控制（作者译）
Acta Histochem Suppl. 1980;22:41-6.
2
[Structure of antibodies and the specificity of the antigen-antibody interaction (author's transl)].[抗体的结构与抗原 - 抗体相互作用的特异性（作者译）]
Acta Histochem Suppl. 1980;22:27-38.
3
4
[Immunofluorescent-histologic localization of a placenta-antigen with antibodies from pregnancy blood (author's transl)].用妊娠血液中的抗体对胎盘抗原进行免疫荧光组织学定位（作者译）
Z Geburtshilfe Perinatol. 1977 Aug;181(4):264-8.
5
6
[The effect of overall charge and pH upon the preparation of anti basement antibodies and upon their capacity of binding in bullous pemphigoid (author's transl)].
Res Exp Med (Berl). 1974 Jul 4;163(3):211-7. doi: 10.1007/BF01851668.
7
[Conjugation and isolation of POD labelled antibodies [author's transl)].
Acta Histochem Suppl. 1980;22:67-71.
8
[Activity and structure of antibody].[抗体的活性与结构]
Tanpakushitsu Kakusan Koso. 1971 Oct;16(11):943-51.
9
[Species-specific antibodies in the immunodiagnosis of malaria (author's transl)].
Tropenmed Parasitol. 1974 Dec;25(4):477-84.
10
[Demonstration of precipitating antibodies against Coxsackie virus type B5, using acetate foil electrophoresis (author's transl)].
Zentralbl Bakteriol Orig A. 1973 Aug;224(3):309-15.
Zotero 插件